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Lysosomal Damage Drives Mitochondrial Proteome Remodelling and Reprograms Macrophage Immunometabolism

Claudio BussiTiaan HeunisEnrica PellegrinoElliott M. BernardNourdine BahMariana Silva dos SantosPierre SantucciBeren AylanAngela RodgersAntony FearnsJulia MitschkeChristopher MooreJames I. MacRaeMaria GrecoThomas ReinheckelMatthias TrostMaximiliano Gutierrez

出版	Universität, 2022
URL	http://books.google.com.hk/books?id=00OmzwEACAAJ&hl=&source=gbs_api

註釋

Abstract: Transient lysosomal damage after infection with cytosolic pathogens or silica crystals uptake results in protease leakage. Whether limited leakage of lysosomal contents into the cytosol affects the function of cytoplasmic organelles is unknown. Here, we show that sterile and non-sterile lysosomal damage triggers a cell death independent proteolytic remodelling of the mitochondrial proteome in macrophages. Mitochondrial metabolic reprogramming required leakage of lysosomal cathepsins and was independent of mitophagy, mitoproteases and proteasome degradation. In an in vivo mouse model of endomembrane damage, live lung macrophages that internalised crystals displayed impaired mitochondrial function. Single-cell RNA-sequencing revealed that lysosomal damage skewed metabolic and immune responses in alveolar macrophages subsets with increased lysosomal content. Functionally, drug modulation of macrophage metabolism impacted host responses to Mycobacterium tuberculosis infection in an endomembrane damage dependent way. This work uncovers an inter-organelle communication pathway, providing a general mechanism by which macrophages undergo mitochondrial metabolic reprograming after endomembrane damage