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Opsonization-independent Antigen-specific Recognition by Myeloid Phagocytes Expressing Monoclonal Antibodies

Michael NeumaierSophie GieslerVolker AstMathis RömerTimo-Daniel VoßEileen ReinzVictor CostinaMartin SchmelzElina NürnbergStefanie NittkaAino-Maija LeppäRüdiger RudolfAndreas TrumppTina Fuchs

出版	Universität, 2023
URL	http://books.google.com.hk/books?id=1qRR0AEACAAJ&hl=&source=gbs_api

註釋Abstract: This report demonstrates a novel class of innate immune cells designated "variable immunoreceptor-expressing myeloids" (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell surface signatures and regularly circulate in the blood of healthy individuals. Single-cell data reveal clonal expansion of circulating B-VIREMs as a dynamic response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport to the cell surface. A first cloned B-VIREM-derived antibody (Vab1) specifically binds stomatin, a ubiquitous scaffold protein that is strictly expressed intracellularly, allowing Vab1-bearing macrophages to phagocytose cell debris without requiring prior opsonization. Our results suggest important antigen-specific tissue maintenance functionalities in these innate immune cells