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Developing a Diagnostic Tool for Acyl Carrier Proteins Through Trypsinolysis, Reverse-phase Chromatography and Native Chemical Ligation

Graciela Reyes

出版	University of Texas, 2010
URL	http://books.google.com.hk/books?id=32ZyAQAACAAJ&hl=&source=gbs_api

註釋

Polyketide biosynthesis is a field that has had tremendous advances in the past 50 years. The understanding of the mechanisms is updated as investigations delve into domain interactions of these microbial natural products. Although numerous polyketides are known, similarities in the sequence of product generation can be used as templates for further exploration of enzymatic activity. The focus of studies recently has been towards developing protocols to manipulate the natural products resulting in medicinally important manufactured products. This investigation examined the mechanism of the acyl carrier protein (ACP) module involved in biosynthesis.