登入
選單
返回
Google圖書搜尋
Digital DNA Microarray Generation on Glass Substrates
Johannes Wöhrle
Stefan Daniel Krämer
Philipp A. Meyer
Matthias Hügle
Gerald A. Urban
Christin Rath
Günter Roth
出版
Universität
, 2020
URL
http://books.google.com.hk/books?id=3B18zgEACAAJ&hl=&source=gbs_api
註釋
Abstract: In this work we show how DNA microarrays can be produced batch wise on standard microscope slides in a fast, easy, reliable and cost-efficient way. Contrary to classical microarray generation, the microarrays are generated via digital solid phase PCR. We have developed a cavity-chip system made of a PDMS/aluminum composite which allows such a solid phase PCR in a scalable and easy to handle manner. For the proof of concept, a DNA pool composed of two different DNA species was used to show that digital PCR is possible in our chips. In addition, we demonstrate that DNA microarray generation can be realized with different laboratory equipment (slide cycler, manually in water baths and with an automated cartridge system). We generated multiple microarrays and analyzed over 13,000 different monoclonal DNA spots to show that there is no significant difference between the used equipment. To show the scalability of our system we also varied the size and number of the cavities located in the array region up to more than 30,000 cavities with a volume of less than 60 pL per cavity. With this method, we present a revolutionary tool for novel DNA microarrays. Together with new established label-free measurement systems, our technology has the potential to give DNA microarray applications a new boost