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Separation of Forskolin Derivatives by Automated Multiple Development High-performance Thin-layer Chromatography
註釋An optimized gradient enabling the separation and quantification of eleven forskolin derivatives by automated multiple development on high-performance thin-layer chromatography (HPTLC) plates is presented. The HPTLC development uses a 25-step gradient with a polarity range of methylene chloride-methanol to hexane and detection by chlorosulfonic acid reagent. Multiple correspondence analysis has allowed correlation of the molecular structures of forskolin derivatives (with hydroxyl substitutions) to the migration distance.