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Simultaneous Detection of Multiple STR Loci on Sex Chromosomes for Forensic Testing of Sex and Identity
Z. Tun
K. Honda
M. Nakatome
M. Nakamura
S. Shimada
Y. Ogura
H. Kuroki
M. Yamazaki
M. Terada
R. Matoba
出版
ASTM International
, 1999
URL
http://books.google.com.hk/books?id=5dt7zgEACAAJ&hl=&source=gbs_api
註釋
The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR.
