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註釋Biohydrogen (H2) production from renewable wastewater signifies a sustainable alternative to a fossil fuel-based economy. H2 is deemed to be the futuristic energy carrier with a high-energy yield. This chapter specifically addresses H2 production through wastewater remediation. A comprehensive emphasis is made on the fermentative routes, namely, light-driven and light-independent processes, encompassing wastewater and mixed culture application as a focal point. Important process parameters that have critical roles on process efficiency are elaborated. Some of the persistent limitations encountered during the process operation are delineated. Stress is made on the pertinent discussion on biocatalyst pretreatment methods in the context of mixed culture wastewater usage. Reported strategies to overcome the limitations are discussed to make the process economically viable, especially for large-scale applications.