Method Development in Analytical HPLC presents the essential information for understanding the process of developing an HPLC method of analysis. It includes foundational information related to HPLC, as well as discussion of sample types, the properties of analytes and matrices in the samples, and sample preparation, which is an important step in analytical methods development. The book provides clear guidance for adopting analytical methods from the literature, informs development of original methods with selection of the suitable type of HPLC, of specific columns, mobile phase, and detection techniques with emphasis on the use of mass spectrometry for detection, as well as optimization and validation of the chosen analytical method. The book also includes useful details on method development for specific types of chromatography such as RP-HPLC, HILIC, ion exchange, size exclusion, and chiral. Method Development in Analytical HPLC also includes information about green chemistry in analytical methods, computer assisted method development, and other key contemporary aspects of the subject.