At the request of the National Institute of Standards and Technology (NIST), the National Academies of Sciences, Engineering, and Medicine has, since 1959, annually assembled panels of experts from academia, industry, medicine, and other scientific and engineering communities to assess the quality and effectiveness of the NIST measurements and standards laboratories, of which there are now seven, as well as the adequacy of the laboratories’ resources. An Assessment of the National Institute of Standards and Technology Center for Nanoscale Science and Technology: Fiscal Year 2016 assesses the scientific and technical work performed by the NIST Center for Nanoscale Science and Technology and the accomplisments, challenges, and opportunities for improvement.