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Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types
Yana Tarakanchikova
Jamal Alzuʾbi
Valentina Pennucci
Marie Follo
Boris Kochergin
Albert Muslimov
Ilya Skovorodkin
Seppo Vainio
Maria N. Antipina
Vsevolod Atkin
Alexey Popov
Igor Meglinski
Anton Cathomen
Tatjana I. Cornu
Dmitry A. Gorin
Gleb B. Sukhorukov
Irina Nazarenko
出版
Universität
, 2020
URL
http://books.google.com.hk/books?id=A12dzQEACAAJ&hl=&source=gbs_api
註釋
Abstract: Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo