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Isolation and Profiling of Viable Tumor Cells from Human Ex Vivo Glioblastoma Cultures Through Single-cell Transcriptomics

Junyi ZhangJakob SträhleKevin JosephNicolas NeidertSimon P. BehringerJonathan M. GöldnerAndreas VlachosMarco PrinzChristian FungJürgen BeckOliver SchnellDieter Henrik HeilandVidhya M. Ravi

出版	Universität, 2023
URL	http://books.google.com.hk/books?id=Bmoj0AEACAAJ&hl=&source=gbs_api

註釋

Abstract: Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level.

For complete details on the use and execution of this protocol, please refer to Ravi et al.1