登入選單

返回Google圖書搜尋

Enhanced Differentiation of Functional Human T Cells in NSGW41 Mice with Tissue-specific Expression of Human Interleukin-7

Emilie CoppinBala Sai SundarasettySusann RahmigJonas BlumeNikita A. VerheydenFranz BahlmannSarina RavensUndine SchubertJanine SchmidStefan LudwigConstantin von KaisenbergAlexander PlatzRonald NaumannBarbara LudwigImmo PrinzClaudia WaskowAndreas Krueger

出版	Springer Nature, 2020
URL	http://books.google.com.hk/books?id=DDQi0AEACAAJ&hl=&source=gbs_api

註釋

Umanized mouse models have become increasingly valuable tools to study human hematopoiesis and infectious diseases. However, human T-cell differentiation remains inefficient. We generated mice expressing human interleukin-7 (IL-7), a critical growth and survival factor for T cells, under the control of murine IL-7 regulatory elements. After transfer of human cord blood-derived hematopoietic stem and progenitor cells, transgenic mice on the NSGW41 background, termed NSGW41hIL7, showed elevated and prolonged human cellularity in the thymus while maintaining physiological ratios of thymocyte subsets. As a consequence, numbers of functional human T cells in the periphery were increased without evidence for pathological lymphoproliferation or aberrant expansion of effector or memory-like T cells. We conclude that the novel NSGW41hIL7 strain represents an optimized mouse model for humanization to better understand human T-cell differentiation in vivo and to generate a human immune system with a better approximation of human lymphocyte ratios.