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Characterization of BHLH-PAS Superfamily of Transcription Factors

Sanjay Jain

出版	Northwestern University, 1999
URL	http://books.google.com.hk/books?id=FTIdAQAAMAAJ&hl=&source=gbs_api

註釋

In the latter years of this study it was discovered that the ARNT protein also mediates hypoxia signaling via its interaction with two PAS homologues, HIF1alpha (MOP1) and HIF2alpha (MOP2). This finding raised the possibility of cross-talk among the dioxin and hypoxia pathways. Therefore, we performed detailed analyses of the ontogeny of the known bHLH-PAS proteins in the mouse. We did this for two main reasons---(1) to identify the potential biological sites where cross-talk between dioxin and hypoxia pathways exist, and (2) to gain insights into developmental roles of these proteins. Using ribonuclease protection assays and cRNA in situ hybridization experiments we determined the ontogenic expression patterns of AHR, ARNT, ARNT2, HIF1alpha and HIF2alpha. Our results identified the putative biological cross-talk sites in murine tissues during development and indicated that there is limited redundancy in the expression patterns of the various homologues.