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Cloning of a Putative Chicken Clock Ortholog and Alteration of a Circadian Rhythm, in Vitro, by Overexpression of a CLOCK Paralog, NPAS2

Kenneth Joseph Seidenman

出版	Northwestern University, 1998
URL	http://books.google.com.hk/books?id=FuZIAAAAYAAJ&hl=&source=gbs_api

註釋

The mouse basic helix-loop-helix-PAS (bHLH-PAS) transcription factor Clock and its Drosophila ortholog have both been shown to play an essential role in circadian timing. The cloning of an avian ortholog of the mammalian Clock gene was therefore undertaken with the goal of providing an entry point for the molecular analysis of the chick pineal circadian system. A full length Gallus homolog of Clock, which we term cClock was isolated by PCR amplification and subsequent screening of chick pineal cDNA libraries. cClock mRNA expression is widely distributed throughout the organism with a pattern similar to that observed in the mouse. No significant circadian differences in cClock mRNA levels were detected in the pineal gland or in pineal cell cultures, consistent with the lack of circadian regulation of the expression of this gene in mammals. Based on the high level of conservation between Clock and cClock and their similar expression patterns, it is likely that cClock is the chicken ortholog of the Clock gene.