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Analysis of Mdm38 Function in Mitochondrial Translation

Jan-Moritz Wuttke

出版	Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015
URL	http://books.google.com.hk/books?id=Gj9FjwEACAAJ&hl=&source=gbs_api

註釋

The data presented in this thesis demonstrate that binding of Mdm38 to ribosomes has conserved characteristics in yeast and E. coli. To gain further insights into potential binding partners, an in vivo photo cross-linking assay was established. Here, preliminary mass spectrometry analysis of a specific cross-linked product revealed proteins involved in pre- and post-translational steps. Nevertheless, further studies are required to improve purifications and to enable the analysis of lower molecular weight proteins. Further, it was shown that a fusion of the biotin ligase, BirA, to Mdm38 can ...