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Animal Cell Culture

其他書名	A Manual of Basic Techniques
出版	Educreation Publishing, 2018-08-10
主題	Education / General
URL	http://books.google.com.hk/books?id=JWdmDwAAQBAJ&hl=&source=gbs_api
EBook	SAMPLE

註釋The cell and tissue culture has become one of the key and foremost tools used in the life sciences today. It plays pivotal and an enormous role and its applications are increasing day by day at an alarming rate. This hand book serves as a guide and is designed to serve as a basic introduction to animal cell culture. It is a right path for laboratory workers who are using it for the first time, as well as for those who interact with cell culture researchers and who want a better understanding of the key concepts and terminology in this interesting and rapidly growing field. The handbook is lucid, covering topics such as getting familiar with the requirements of a laboratory dedicated to cell culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, freezing, and thawing cultured cells. The information and guidelines presented in the handbook focus on cell lines (finite or continuous) and omit experiments and techniques concerning primary cultures such as isolating and disaggregating tissues. Note: The basics of cell culture experiments share certain similarities, cell culture conditions vary widely for each cell type. Deviating from the culture conditions required for a particular cell type can result in different phenotypes being expressed, therefore you should be familiar with the cell line you are of interest, and closely follow the instructions provided with each product you are using in your experiment.