Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
- Have you selected the most appropriate research strategy?
- Have you identified the issues critical to your successful application of a technique?
- Are you familiar with the limitations of a given technique?
- When should common procedural rules of thumb not be applied?
- What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
- Working safely with biological samples and radioactive materials
- DNA and RNA purification
- PCR
- Protein and nucleid acid hybridization
- Prokaryotic and eukaryotic expression systems
- Properly using and maintaining laboratory equipment
