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Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques
Robert E. Levin
出版CRC Press, 2009-10-26
主題Science / Life Sciences / MicrobiologyTechnology & Engineering / Food Science / GeneralScience / Life Sciences / Molecular Biology
ISBN142009243X9781420092431
URLhttp://books.google.com.hk/books?id=L-27CodAfYMC&hl=&source=gbs_api
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註釋Written by one of the most prolific and respected researchers in food safety, this volume describes molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. Each chapter deals with a specific organism and techniques applied to that organism. Particular focus is placed on genes associated with pathogenicity used in the polymerase chain reaction (PCR) including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. Methods for extracting microorganisms from complex food matrices andDNA purification techniques are also emphasized.