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Polyadenylation of Messenger RNA and Termination of Transcription in Polyomavirus [microform]

Jacqueline Lanoix Bergeron

出版	Thesis (Ph.D.)--McGill University, 1988
ISBN	03154855909780315485594
URL	http://books.google.com.hk/books?id=LqumSgAACAAJ&hl=&source=gbs_api

註釋

Circumstantial evidence suggested that polyadenylation and termination of transcription may be linked. To test this hypothesis, we constructed viable insertion mutants of polyomavirus that contain a second functional polyadenylation signal. We showed that the polyadenylation of L-strand RNAs requires sequence elements located between 12 and 87 nucleotides downstream of AAUAAA. Polyadenylation ofE-strand RNAs requires no more than 19 nucleotides upstream and 44 nucleotides downstream of AAUAAA. An insertion mutant (ins 4) that contained a second functional L-strand signal produced L-strand RNAs with 3' ends at both sites. However, the efficiency of polyadenylation and that of transcription termination remained as low as that in wild type-infected cells. [...].