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Quantifying Active Endothelial Cell Receptor-ligand Binding as a Function of Engineered Surfaces

出版	University of Florida, 2002
URL	http://books.google.com.hk/books?id=OA8OkAEACAAJ&hl=&source=gbs_api

註釋ABSTRACT (cont.): The selectins formed clusters at the periphery of the PVECs. The sLeX-selectin interaction was found to have a binding force of 127 +/- 45 pN and an interaction distance of 28 +/- 9 nm for PVECs cultured on polystyrene. Novel polymer surfaces with engineered topographies were fabricated using a combination of micrometer scale photolithography overlaid with nanometer scale AFM based nanolithography. This resulted in epoxy and PDMS elastomer surfaces with a hierarchy of topography similar in scale to extracellular matrix. This research has demonstrated the use of the AFM to characterize the physical and mechanical properties of polymer surfaces. Through chemical functionalization of the tip, it is the first known example of quantitative analysis of receptor-ligand binding on living cells. Concurrently, it is also the first known example of mapping the location of the cell surface receptors with micrometer resolution on living cells. The capability demonstrated here is critical in evaluating cellular response to biomaterials by examining expression of proteoglycans on the cell surface. By introducing a novel approach to creating hierarchical topographies to simulate extracellular matrix, this research presents a fabrication process to direct cell functions. In the near future, combination of quantitative analysis of cellular responses and nanometer scale engineered surfaces will lead to optimized biointerfaces for medical device coatings and tissue engineering constructs.