返回Google圖書搜尋

EPB41L5 Controls Podocyte Extracellular Matrix Assembly by Adhesome-dependent Force Transmission

Jasmin I. MaierManuel RoggMartin HelmstädterAlena SammarcoOliver SchillingBenedikt SabassJeffrey H. MinerJörn DengjelGerd WalzMartin WernerTobias HuberChristoph Schell

出版	Universität, 2021
URL	http://books.google.com.hk/books?id=OAyCzgEACAAJ&hl=&source=gbs_api

註釋Abstract: The integrity of the kidney filtration barrier essentially relies on the balanced interplay of podocytes and the glomerular basement membrane (GBM). Here, we show by analysis of in vitro and in vivo models that a loss of the podocyte-specific FERM-domain protein EPB41L5 results in impaired extracellular matrix (ECM) assembly. By using quantitative proteomics analysis of the secretome and matrisome, we demonstrate a shift in ECM composition characterized by diminished deposition of core GBM components, such as LAMA5. Integrin adhesome proteomics reveals that EPB41L5 recruits PDLIM5 and ACTN4 to integrin adhesion complexes (IACs). Consecutively, EPB41L5 knockout podocytes show insufficient maturation of integrin adhesion sites, which translates into impaired force transmission and ECM assembly. These observations build the framework for a model in which EPB41L5 functions as a cell-type-specific regulator of the podocyte adhesome and controls a localized adaptive module in order to prevent podocyte detachment and thereby ensures GBM integrity