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Separation of Glyoxylated Hemoglobin with Varying Oxygen Affinity by Polyanion Fast Protein Liquid Chromatography

J. Carleton Hsia

出版	Elsevier Science Publishers, 1984
URL	http://books.google.com.hk/books?id=OHBKjwEACAAJ&hl=&source=gbs_api

註釋

Considerable research effort has recently been directed towardthe modification of stroma-free hemoglobin (SFH) to allow its useas a basis for blood substitutes. In this communication, a fastprotein liquid chromatographic (FPLC) method is described whichseparates optimally glyoxylated hemoglobin from a mixture ofnative and partially glyoxylated hemoglobin. The hemoglobinfractions were characterized by their oxygen-binding affinity andtheir electrophoretic mobility on cellulose acetate.