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註釋In combating global warming and other environmental issues over the use of fossil fuels, extensive research has been focusing on developing hydrogen production from biological processes. Biohydrogen is considered a promising future biofuel because of its intrinsic clean and high-energy content properties and the way it is produced. In addition to being produced through environmentally friendly biological means, its conversion to energy yields only pure water, which is an ideal energy carrier in reducing greenhouse gas emissions from fossil fuel combustion. Unlike other well-developed biofuels such as bioethanol and biodiesel, biohydrogen production is still in the early stage of development. A variety of technologies are being developed for biohydrogen production. This chapter presents a review of the state-of-the-art and perspectives of bioprocess design for biohydrogen production research in the context of pathways, microorganisms, metabolic flux analysis, process design, and reactor system. Challenges and prospects of biohydrogen production are also outlined.