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Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-care Compatible QPCR Assay

Pune Nina TawakoliChristopher HerzJoël S. JenzerDaniel B. WiedemeierThomas AttinNagihan BostanciGeorgios N. BelibasakisKai BaoPhilipp KörnerTanja FritzJulia PrinzPatrick Roger SchmidlinThomas ThurnheerFlorian J. WegehauptKonstantinos MitsakakisJohannes R. Peham

出版	Universität, 2020
URL	http://books.google.com.hk/books?id=PFd1zgEACAAJ&hl=&source=gbs_api

註釋Abstract: Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, T. denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycetemcomitans, S. mutans, S. sobrinus, oral associated Lactobacilli) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: C. rectus, T. forsythia, P. gingivalis, S. mutans, F. nucleatum, T. denticola, P. intermedia and oral Lactobacilli (p