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Liquid Chromatography
註釋Derivatization employs various microchemical reactions to alter the structure of the analyte to facilitate its isolation, separation or detection. Reactions are commonly performed either off-line and prior to the separation or on-line and after the separation and prior to detection. Reagents and reaction conditions are suggested for improved uv-visible detection, fluorescence and chemiluminescence detection, electrochemical detection, mass spectrometric detection, separation of enatiomers, formation of cyclic derivatives, and solid-phase analytical derivatization. The design of reactors for post-column reactions are described for continuous detection in an automated mode.