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Development and characterization of novel reversibly switchable red fluorescent proteins with opposing switching modes
Isabelle Jansen
出版
Georg-August-Universität Göttingen
, 2020
URL
http://books.google.com.hk/books?id=TqNy0AEACAAJ&hl=&source=gbs_api
註釋
Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a multitude of fluorescent proteins have been derived from the original GFP and its homologs by protein engineering. Their characteristics were especially adapted to the requirements of different types of live-cell fluorescence microscopy. This includes super-resolution microscopy techniques, which overcome the diffraction barrier by distinguishing fluorophores based on different molecular states. Fluorescent proteins in the subclass of reversibly switchable fluorescent proteins (RSFPs) can be s...