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Fetal Calf Serum Alternatives for Application in Tissue Engineering

出版	2017
URL	http://books.google.com.hk/books?id=U36vzQEACAAJ&hl=&source=gbs_api

註釋IntroductionTissue engineering is an emerging field with growing importance in oncological reconstructive surgery, as it may help to replace damaged or injured body tissue with compatible substitutes. For overcoming initial loss of the tissue engineered substitutes, pre-vascularized tissue surrogates would be helpful to enable a rapid/fast/quick connection of the blood vessels of the recipient. In vitro, culture of tissue engineered body substitutes are performed with FCS (fetal calf serum). Nevertheless, for in vivo application in humans, human platelet lysates may successfully replace FCS.MethodologyHuman umbilical vein endothelial cells (HUVEC) were cultured with fetal calf serum and with two commercially available human platelet lysates (MultiPlu00b430 and MultiPLu00b4100, kindly provided by MacoPharma) or one non-commercially available human platelet lysates (hPL) (manufactured by Max Planck Institute for Polymer Research Mainz) in different concentrations. Cell viability, proliferation and tube formation assays were conducted by using platelet lysates and compared with 20 % FCS.ResultProliferation and cell viability of HUVEC cultured for 72 hours with the distinct types of human platelet lysates in different concentrations were similar to those of the controls (cells cultured in 20% FCS). Capillary-like structures of HUVEC cultured in all platelet lysates were more effective than cultured in FCS. The induction of tube formation was most effective by using 5% MultipliPLu201930. However, the use of platelet lysates in concentrations between 2.5% and 5% were more effective than the cultivation in 20% FCS. Conclusions To prevent tissue rejection or adverse effects like an allergic reaction, autologous cells should be cultivated with human platelet lysates. We showed that human platelet lysates can replace FCS in the in vitro cultivation process and, additionally, that these cells are able to form capillary-like structures as prerequisite for pre-vascularized tissue substitutes in humans.