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In Vitro Production of Colchiploids and Mutants of Native Florida Diploid Vaccinium Species and Hybrids and Evaluation of 12X V. Ashei Colchiploids

Julia Lucile Wood Perry

出版	University of Florida, 1984
URL	http://books.google.com.hk/books?id=WadyIgAACAAJ&hl=&source=gbs_api

註釋

In vitro produced colonies of Florida native diploid blueberry species Vaccinium elliottii Chapm., V. darrowi Camp, and their interspecific hybrid were treated with colchicine to double their chromosome number and facilitate interspecific hybridization with tetraploid highbush blueberry, V. corymbosum Lamarck. In vitro colchiploids, identified by increased shoot diameter, were confirmed as tetraploid by stomate guard cell measurement and chromosome counts from shoot tip squashes. V. elliottii produced the greatest number of increased-diameter shoots when 2-node cuttings were treated for 48 hours on a rotary drum in liquid modified Knops medium containing 0.1% colchicine, or for 2-6 weeks without agitation on solid modified Knops medium containing 0.01% colchicine. V. darrowi produced the greatest number of increased-diameter shoots when 2-node cuttings were treated on a rotary drum in .01% colchicine-containing liquid modified Knops medium for 24 hours. The V. elliottii X V.