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The Green Alga Chlamydomonas Reinhardtii

其他書名	A New Model System to Unravel the Biogenesis of Respiratory Complexes
出版	Ohio State University, 2010
URL	http://books.google.com.hk/books?id=Xx5QAQAACAAJ&hl=&source=gbs_api

註釋Chapter 2 shows the use of Chlamydomonas reinhardtii as a forward genetics tool to screen for Complex I deficiency mutants and the finding of six new loci that result in defects in assembly. Based on large level of conservation of Complex I subunits in eukaryotes as well as the presence of homologs of currently proposed assembly factors, this work follows the hypothesis that the assembly process is also conserved among eukaryotes. Chapter 3 refers to the molecular identification of one of these genes as the Complex I subunit NUOB10, homolog of the bovine PSDW subunit. Also, a molecular mapping approach for another mutant suggests that the mutation does not affect a structural subunit of Complex I and that the final identification of this gene will lead to the discovery of a novel assembly factor. Finally, Chapter 4 provides an initial evaluation for the overall functional conservation of two known assembly factors: CIA30 and OXA1, both of which have a ortholog gene in Chlamydomonas.