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Distributions of Allelic Frequencies and Haplotypes of Three New Y-STR Loci in a Chinese Han Population

HJ. ZhangYH. ShenQF. ZhuQH. WangQ. JiJP. TangJ. LiaoYG. LinYP. Hon

出版	ASTM International, 2006
URL	http://books.google.com.hk/books?id=ZWJ5zgEACAAJ&hl=&source=gbs_api

註釋

Whole blood samples were collected from 109 unrelated males of Han ethnic group in Sichuan of China. DNA was extracted using Chelex method (1) The volume of PCR reaction for each locus was 25 μL, which contained 1-5 ng human genome, 1 X Taq buffer, 1.5 mM MgCl2, 200 R M each dNTP (Pharmacia Biotech, Sweden), 1 U Taq polymerase (Applied Biosystems, Foster City, CA), 0.25 R M each primer (Each forward primer was 5' FAM labeled) PCR amplifications were carried out in a GeneAmp PCR System 9600 (Perkin-Elmer, Foster City, CA) The cycling protocols were: 94°C for 1 min, followed by 30 cycles of 94°C for 30 s, 59°C for 30 s, 72°C for 40 s, and extension at 72°C for 30 min. Typing were performed using denaturing capillary gel electrophoresis on an ABI PRISM 310 Genetic Analyzer with laser-induced fluorescence detection. Alleles were designated according to recommendation of the DNA commission of the International Society of Forensic.