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出版Universität, 2017
URLhttp://books.google.com.hk/books?id=ZcOVzgEACAAJ&hl=&source=gbs_api
註釋Abstract: Cell ReportsArticleLSD1 Controls TimelyMyoDExpressionvia MyoD Core Enhancer TranscriptionIsabella Scionti,1,2Shinichiro Hayashi,3,4Sandrine Mouradian,1,2Emmanuelle Girard,1,2,9Joana Esteves de Lima,3Ve ́ronique Morel,1,2Thomas Simonet,1,2Maud Wurmser,5Pascal Maire,5Katia Ancelin,1Eric Metzger,6,7,8Roland Sch€ule,6,7,8Evelyne Goillot,1,2,*Frederic Relaix,3and Laurent Schaeffer1,2,9,10,*1Institut NeuroMyoGene, CNRS UMR5310, INSERM U1217, Universite ́Lyon1, 46 Alle ́e d'Italie, 69007 Lyon, France2Laboratory of Molecular Biology of the Cell, CNRS UMR5239, Universite ́Lyon 1, ENS Lyon, 46 Alle ́e d'Italie, 69007 Lyon, France3Biology of the Neuromuscular System, INSERM IMRB-E10 U955, Universite ́Paris-Est, 8 rue du Ge ́ne ́ral Sarrail, 94010 Cre ́teil Cedex, France4Department of Cellular and Molecular Medicine, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45 Yushima,Bunkyo-ku, Tokyo 113-8510, Japan5Institut Cochin, INSERM U1016, CNRS UMR 8104, Universite ́Paris Descartes, Sorbonne Paris Cite ́, 22 rue Mechain, 75014 Paris, France6Klinik f€ur Urologie und Zentrale Klinische Forschung, Klinikum der Universita ̈t Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany7Deutsches Konsortium f€ur Translationale Krebsforschung, Standort Freiburg, 79106 Freiburg, Germany8BIOSS Centre of Biological Signalling Studies, Albert Ludwigs University Freiburg, 79106 Freiburg, Germany9Hospices Civils de Lyon, Faculte ́de Medicine Lyon Est, 3 Quai des Ce ́lestins, 69002 Lyon, France10Lead Contact*Correspondence:evelyne.goillot@ens-lyon.fr(E.G.),laurent.schaeffer@univ-lyon1.fr(L.S.)http://dx.doi.org/10.1016/j.celrep.2017.01.078SUMMARYMyoDis a master regulator of myogenesis.Chromatin modifications required to triggerMyoDexpression are still poorly described. Here, wedemonstrate that the histone demethylase LSD1/KDM1a is recruited on theMyoDcore enhancerupon muscle differentiation. Depletion ofLsd1inmyoblasts precludes the removal of H3K9 methyl-ation and the recruitment of RNA polymerase II onthe core enhancer, thereby preventing transcriptionof the non-coding enhancer RNA required forMyoDexpression (CEeRNA). Consistently,Lsd1condi-tional inactivation in muscle progenitor cells duringembryogenesis prevented transcription of theCEeRNA and delayedMyoDexpression. Our resultsdemonstrate that LSD1 is required for the timelyexpression ofMyoDin limb buds and identify a newbiological function for LSD1 by showing that it canactivate RNA polymerase II-dependent transcriptionof enhancers