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Cyclic Stretch Increases Splicing Noise Rate in Cultured Human Fibroblasts

Michael UhlKevin Marcel MellertBritta StrieglMartin DeiblerMarkus LamlaJoachim P. SpatzRalf KemkemerDieter Kaufmann

出版	Universität, 2011
URL	http://books.google.com.hk/books?id=_T-mzwEACAAJ&hl=&source=gbs_api

註釋Abstract: Background
Mechanical forces are known to alter the expression of genes, but it has so far not been reported whether they may influence the fidelity of nucleus-based processes. One experimental approach permitting to address this question is the application of cyclic stretch to cultured human fibroblasts. As a marker for the precision of nucleus-based processes, the number of errors that occur during co-transcriptional splicing can then be measured. This so-called splicing noise is found at low frequency in pre-mRNA splicing.

Findings
The amount of splicing noise was measured by RT-qPCR of seven exon skips from the test genes AATF, MAP3K11, NF1, PCGF2, POLR2A and RABAC1. In cells treated by altered uniaxial cyclic stretching for 18 h, a uniform and significant increase of splicing noise was found for all detectable exon skips.

Conclusion
Our data demonstrate that application of cyclic stretch to cultured fibroblasts correlates with a reduced transcriptional fidelity caused by increasing splicing noise