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Caldicellulosiruptor Obsidiansis Sp. Nov.,
其他書名
An Anaerobic, Extremley Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park
出版American Society for Microbiology, 2009
URLhttp://books.google.com.hk/books?id=acP_zgEACAAJ&hl=&source=gbs_api
註釋A novel, obligately anaerobic, extremely thermophilic, cellulytic bacterium, designations OB47T, was isolated from Obsidian Pool, Yellowstone National Park, WY. The isolate was a nonmotile, non-spore-forming, Gram-positive rod approximately 2 ?.m. long by 0.2 ?.m. wide and grew at temperatures between 55 and 85[degrees]C, with the optimum at 78[degress]C. The pH range for growth was 6.0 to 8.0, with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rate at 0.75 h[superscript -1]. The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactin, Avicel, xylan, filter paper, processed cardboard, pectin, dilute, acid-pretreated switchgrass, and Populus. OB47T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbitol, carboxymethylcellulose, and casein. Yeast extract stimulated growth, and thiosulfate, sulfate, nitrate, and sulfur were not reduced. Fermentation end products were mainly acetate, [hydrogen], and [carbon dioxide], although lactate and ethanol were produced in 5-liter batch fermentations. The G+C content of the DNA was 35 mol%, and sequence analysis of the small subunit rRNA gene placed OB47T within the genus Caldicellulosiruptor obsidiansis sp. nov. and OB47 is the type strain (ATCC BAA-2073).