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Tolerability and Cell Yield from Nasal Biopsies from Obtained in the Outpatient Setting

出版	2017
URL	http://books.google.com.hk/books?id=afezzQEACAAJ&hl=&source=gbs_api

註釋Title: tTolerability and cell yield from nasal biopsies obtained in the outpatient settingAuthors:tMark McClure1, Lucy A. Truman2, Marcos Martinez Del Pero, David Jayne11 Department of Medicine, Addenbrookes Hospital, Cambridge, UK2 ENT Department, Addenbrookes Hospital, Cambridge, UKSubject Area:Rhinology/Anterior Skull Base Surgery or General ENT Background:tFluorescence-activated cell sorting (FACS) enables accurate immunophenotyping of lymphocytes from small amounts of tissue.Objectives:tWe assessed the cell yield and tolerability of biopsies taken from the nose under topical anaesthetic in the outpatient setting. Methods:7 patients with flaring granulomatosis with polyangiitis consented to biopsies. A mixture of Lidocaine hydrochloride 5% and phenylephrine 0.5% was applied topically. Biopsies were taken from the post-nasal space (PNS) the lateral wall and septum. Cells were processed and run on an LSR-Fortessa flow cytometer. Results: 5/7 patients tolerated the procedure well and 6 agreed to return for repeat biopsy. Cell yields were highest from the PNS biopsy site (median 38355, IQR 1002-97185) compared with the lateral wall (1136, IQR 202-2596), septum (236, IQR 175-1030) and inflamed area (197, IQR 72-397). (sd 588). CD3+CD4+ cells (81%) were the dominant T cell and CD19+C27+IgD- (68%) the most frequent B cells.Conclusions: Nasal biopsy was well tolerated in the outpatients setting and cell yields were high enough to accurately examine lymphocyte subsets.