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Characterization of a Mesophilic Anaerobic Inoculum by Means of Methanogenic Activity Tests

出版	Universitat Politècnica de Catalunya. Escola Superior d'Agricultura de Barcelona, 2014 (Grau en Enginyeria dels Sistemes Biològics), 2014
URL	http://books.google.com.hk/books?id=arwIywEACAAJ&hl=&source=gbs_api

註釋The anaerobic digestion is a well-known technology to treat wastes with a high potential of methane recovery (i.e. manures, slaughterhouse waste, animal by-products waste and other types of organic wastes) even presenting a relatively high nitrogen content. However, wastes with high nitrogen content involve several problems on the anaerobic digestion process. Ammonia causes inhibition on aceticlastic methanogens but in lesser extend on Syntrophic Acetate Oxidation microorganisms (SAO) or on hydrogenotrophic methanogens. Thus, working with SAO population it is possible to avoid or to decrease the effects of the inhibition by ammonium. The aim of this project is to characterize a mesophilic anaerobic inoculum likely to have presented SAO activity, in the past, through methanogenic activity tests. In this study, the effect of acetate, hydrogen and ammonia concentrations on the methanogenic activity was elucidated in two experiments; in the first one, an anaerobic mesophilic inoculum was exposed to diverse concentrations of acetate and ammonia in presence of hydrogen, and in the second, a SAO mesophilic inoculum was exposed to a high ammonia and acetate concentrations. The methanogenic activities were determined through sequential batch experiments estimating the acetate and hydrogen consumption rates and also the methane production rate. After a three successive pulses of acetate and hydrogen during 140 days, it was observed a decrease on the hydrogenotrophic activity and a increase on the aceticlastic activity. According to our results and considering that the inoculum came from a reactor characterized by a retention time of 65 days, fed with protein-rich wastes and operated with high content of ammonia nitrogen in the digester, it is possible to hypothesize that the reactor presented SAO activity.