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Root Organogenesis in Medicago Truncatula

出版	Australian National University, 2011
URL	http://books.google.com.hk/books?id=bZYUnQEACAAJ&hl=&source=gbs_api

註釋Plant stem cells are contained within niches like the root apical meristem (RAM) which create new cells for organ growth, plant stem cells may also be initiated post-embryogenesis like for the development of lateral roots. Secondary growth from the meristems of the vascular and cork cambia also occurs in roots and in some species specialised roots are formed for storage, in response to environmental cues and symbiotic and pathogenic microorganisms. Thus root organogenesis provides a widely variable model to examine one of biology's great questions, how can stem cells be created and maintained and how is differentiation specified and controlled? This thesis describes original proteomic and transcriptomic approaches to gene discovery in a variety of root organogenesis systems using the model legume Medicago truncatula. I used proteomics to compare the meristem and differentiating zones from primary and lateral roots and present and examination the effect of meristem inhibiting herbicide acetohydroxyacid synthase on the meristem proteome. I used transcriptomics to characterise the Medicago RAM by comparing the root meristem and non-meristem and extended this microarray analysis further to examine the very early steps of root formation in an in vitro root organogenesis system. These experiments have identified a host of transcription factors that are commonly expressed in root meristems, these genes will have an important role in future studies of the genetic networks controlling plant stem cells in root and nodule organogenesis.