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Biological Processes Induced by ZnO, Amoxicillin, Rye and Fructooligosaccharides in Cultured Intestinal Porcine Epithelial Cells
Marcellus Maria Hulst
Arjan Hoekman
Ilonka Wijers
Dirkjan Schokker
Marinus Adrianus Smits
其他書名
VDI-4 ; In-vitro Tests 2013-2014
出版
Wageningen UR Livestock Research
, 2015
URL
http://books.google.com.hk/books?id=bsPLjgEACAAJ&hl=&source=gbs_api
註釋
The objective of this study was to develop an in-vitro bioassay using cultured Intestinal Porcine Epithelial Cells (IPEC-J2) and evaluate the capability of this assay to predict enterocyte-specific physiological and immunological processes induced by nutrients/additives in the intestines of farm animals. Responses to five nutrients/feed-additives, similar to those studied in animal trials, performed in the Feed4Foodure framework, were measured by gene expression analysis of IPEC-J2 cells either under stressed (Salmonella) or non-stressed conditions. Response genes were analysed using bioinformatics web-tools in order to identify dominant biological processes induced by these nutrients/feed-additives and to predict key-genes/proteins important for regulation of these biological proc
