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Evolutionary Conservation of the Fidelity of Transcription
Claire Chung
Bert M. Verheijen
Zoe Navapanich
Eric G. McGann
Sarah Shemtov
Guan-Ju Lai
Payal Arora
Atif Towheed
Suraiya Haroon
Agnes Holczbauer
Sharon Chang
Zarko Manojlovic
Stephen Simpson
Kelley W. Thomas
Craig Kaplan
Peter M. van Hasselt
Hermanus T. Timmers
Dorothy Erie
Lin Chen
Jean-Franćois Gout
Marc Vermulst
出版
Universität
, 2023
URL
http://books.google.com.hk/books?id=c6MV0AEACAAJ&hl=&source=gbs_api
註釋
Abstract: Accurate transcription is required for the faithful expression of genetic information. However, relatively little is known about the molecular mechanisms that control the fidelity of transcription, or the conservation of these mechanisms across the tree of life. To address these issues, we measured the error rate of transcription in five organisms of increasing complexity and found that the error rate of RNA polymerase II ranges from 2.9 × 10−6 ± 1.9 × 10−7/bp in yeast to 4.0 × 10−6 ± 5.2 × 10−7/bp in worms, 5.69 × 10−6 ± 8.2 × 10−7/bp in flies, 4.9 × 10−6 ± 3.6 × 10−7/bp in mouse cells and 4.7 × 10−6 ± 9.9 × 10−8/bp in human cells. These error rates were modified by various factors including aging, mutagen treatment and gene modifications. For example, the deletion or modification of several related genes increased the error rate substantially in both yeast and human cells. This research highlights the evolutionary conservation of factors that control the fidelity of transcription. Additionally, these experiments provide a reasonable estimate of the error rate of transcription in human cells and identify disease alleles in a subunit of RNA polymerase II that display error-prone transcription. Finally, we provide evidence suggesting that the error rate and spectrum of transcription co-evolved with our genetic code