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Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter Jejuni Bacteria

Dimitra N. Stratis-CullumSun McMastersPaul M. Pellegrino

出版	Army Research Laboratory, 2009
URL	http://books.google.com.hk/books?id=ceh5AQAACAAJ&hl=&source=gbs_api

註釋

In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets.