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出版Google Book Publishers
主題Science / BiotechnologyScience / Life Sciences / Genetics & Genomics
URLhttp://books.google.com.hk/books?id=fJVyEAAAQBAJ&hl=&source=gbs_api
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註釋The present book Microbes in Indian Buffalo Rumen: A Metagenomics Approach will be useful to researchers, who are engaged in research on DNA sequencing of Microbes with diet composition, gut flora, ruminal microbes, and microbial community dynamics of cattle. The book covers not only the rumen metagenomics, but also highlights the salient findings about specifically exploring the microbial community dynamics from Mehsani and Kankrej breeds of buffaloes at different diet treatments, comparing the rumen microbiota at each diet treatments, and identifying the methanogens and their enzymes responsible for methanogenesis at each dietary treatment. The rumen microbiota studied under the different diet treatments consisted of 48 metagenomes per animal species, outputted a huge sequence dataset, the rarefaction analysis of which confirmed the rumen metagenomes were sequenced to sufficient depths. Though Bacteroidetes and Firmicutes appeared as a key role player in the constitution of the ruminal environment, the significant shift in the abundance of the rest minor phyla confirms their auxiliary role in metabolizing of the fibre-rich feed particles. The more occurrences of Clostridium, Ruminococcus and Eubacterium coming from Firmicutes group, in solid fraction as compared to liquid fraction provides the hint of their foremost site of metabolic action, a fibre, where they actively participate in fibre degradation and produces oligo- and di-saccharides (soluble) for further utilization by Bacteroidetes in liquid fraction. Metabolic subsystems shows predominance of carbohydrate utilization among rest of the subsystems provides and thus provides the insights in functional capability of the ruminal microbiota for the degradation of the ingested food and hence aid in endowment of the nutrition to the host. Further, the results of CAZyme families conceived a fact that in Mehsani buffalo, the green roughage showed more abundance of GH2 genes (β-galactosidase) and dry roughage showed more abundance of GH3 genes (exo acting β-glucosidase). Thereby confirming efficient cellulosic biomass degradation in dry roughage as compared to green roughage, where more hydrolysis of lactose as well as the transgalactosylation of galacto-oligosaccharides takes place. Apart from that, the taxonomic assignment of the CAZymecontigs in Kankrej cattle revealed that carbohydrate esterases (CE) are group of the enzymes that majorly contributed by Prevotella, Bacteroides and Parabacteroides, on the other hand, glycosyl hydrolase 3 (GH3), a CAZy family, contributed by Clostridium and Fibrobacter in liquid portion and Clostridium, Eubacterium and Ruminococcus in solid fraction. Moreover, the present book will be very much benefited to all young as well as professional researchers and scientists of India and world over, covering wide regional, national, continental, and global spectrum.