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Utility of the Cytochrome Oxidase I (COI) for Species Identification and Phylogeographic Analysis in Black Flies (Diptera: Simuliidae).

出版	Library and Archives Canada = Bibliothèque et Archives Canada, 2008
ISBN	04945882769780494588277
URL	http://books.google.com.hk/books?id=fRuFYgEACAAJ&hl=&source=gbs_api

註釋A short sequence of ca. 658-bp of the mitochondrial gene COI was used to investigate its utility as a DNA barcode in the medically important Simuliidae or black flies. Sixty-five species and species complexes were tested. Results indicate that the barcoding gene correctly discriminated among morphologically distinct species with nearly 100% of efficacy and also proved useful for revealing cryptic diversity. The DNA barcoding gene was also tested for revealing phylogeographic patterns in the western cordilleran Prosimulium travisi and the Prosimulium neomacropyga species-group. Phylogeographic analyses on these species revealed the areas that acted as glacial refugia, postglacial history, cryptic speciation episodes and timing of the events that lead to their present-day distribution. The results obtained largely concur with other phylogeographic studies on similarly-distributed cordilleran organisms. In conclusion, the barcoding gene not only resulted useful for species discrimination in black flies but also for studies at the population level, providing value-added to this molecular marker.