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Proteomics in Practice
Reiner Westermeier
Tom Naven
Hans-Rudolf Höpker
其他書名
A Guide to Successful Experimental Design
出版
John Wiley & Sons
, 2008-09-08
主題
Science / Life Sciences / Cell Biology
Science / Life Sciences / Biology
Science / Life Sciences / Genetics & Genomics
ISBN
3527622306
9783527622306
URL
http://books.google.com.hk/books?id=h7GLJrKop0IC&hl=&source=gbs_api
EBook
SAMPLE
註釋
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.