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Examination of Glucose Transport in Clostridium Beijerinckii NCIMB 8052 and the Solvent Hyper-Producing Mutant BA101

出版	University of Illinois at Urbana-Champaign, 2003
URL	http://books.google.com.hk/books?id=h8UnjwEACAAJ&hl=&source=gbs_api

註釋Glucose uptake and accumulation by Clostridium beijerinckii BA101, a butanol hyper-producing mutant was examined during various stages of growth. Glucose uptake in C. beijerinckii BA101 was repressed 20% by 2-deoxyglucose (2-DG) and 25% by mannose, while glucose uptake in C. beijerinckii 8052 was repressed 52% and 28% by these sugars, respectively. We confirmed the presence of a phosphoenolpyruvate (PEP) dependent phosphotransferase system (PTS) associated with cell-free extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP. The PTS activity associated with C. beijerinckii BA101 was 50% of that observed for C. beijerinckii 8052. C. beijerinckii BA101 also demonstrated lower PTS activity for fructose and glucitol. Glucose phosphorylation by cell-free extracts derived from both C. beijerinckii BA101 and 8052 was also dependent on the presence of ATP, consistent with the presence of glucokinase activity in C. beijerinckii extracts.^