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Body Water Compartments During Bed Rest

其他書名	Evaluation of Analytical Methods
出版	National Aeronautics and Space Administration, 1973
URL	http://books.google.com.hk/books?id=hCAu_brdfHMC&hl=&source=gbs_api

註釋Nine healthy young men, ages 21 to 25, were studied to determine the reproducibility and interchangeability of the use of (a) radio-iodinated human serum albumin (I131) and Evans Blue dye for estimating plasma volume, (b) sodium bromide for extracellular fluid volume, and (c) deuterium oxide (D2O) for total body water volume. All subjects were tested in a semibasal condition and allowed to rest for at least 30 min after arriving at the laboratory. Evans Blue and I131 were administered intravenously and NaBr and D2) by oral ingestion. Venous blood samples were collected for analysis at various intervals after the tracers were administered. For the measurement of plasma volume, a comparison was made between the 10-min postinjection blood sample and the 0-min value extrapolated from the 10-, 20-, 30-, and 60-min samples. For NaBr and D2O spaces, a comparison was made between the three 3-hr postingestion blood samples and the 0-min value extrapolated from the 2-, 3-, 5-, and 8-hr samples. The results indicate (2) there was uniform distribution of I131 and Evans Blue dye 10 min after injection and of NaBr and D2O 3 hours after oral ingestion; (b) the buildup of residual tracer did not interfere appreciably with the measurement of either I131 and Evans Blue spaces when they were administered at four equal intervals over 8 days or three times in 15 days; and (c) the buildup of background tracer after ingestion of NaBr and D2O once per week for three consecutive weeks did not affect the accuracy of the measurement. Average interindividual variability (±SE) over a 3-week period was ±155 ml for I131 space, ±146 ml for Evans Blue space, ±310 ml for bromide space, and ±2300 ml for total body water space. It was found that (a) I131 and Evans Blue may be used interchangeably for estimating plasma volume and one 10-min equilibration sample gives results comparable to those obtained using the 0-min extrapolation of multiple blood samples, and (b) for estimating bromide and D2O spaces, one 3-hr equilibrium blood sample gives results similar to the extrapolation of multiple samples.