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Pax7 is Required for Muscle Satellite Cell Specification and Regenerative Myogenesis

Patrick Seale

出版	McMaster University, 2003
URL	http://books.google.com.hk/books?id=ijulSgAACAAJ&hl=&source=gbs_api

註釋

Muscle satellite cells are a distinct population of myogenic progenitors that mediate the postnatal growth and regeneration of skeletal muscle. To gain insight into the genetic regulation of satellite cell function during muscle regeneration, genes expressed specifically in these cells were identified by representational difference analysis of cDNAs. Notably, the paired-box transcription factor Pax7 was isolated as a gene specifically expressed in quiescent and activated satellite cells. Cell culture and histological analysis of Pax7-deficient muscle revealed a complete absence of satellite cells. This result demonstrates a requirement for Pax7 upstream of MyoD and Myf5 in the specification of muscle satellite cells. Consistent with their lack of satellite cells, adult Pax7 & minus;/ & minus; mice displayed an aggravated muscle wasting phenotype characterized by spinal kyphosis and reduced muscle mass. Acute muscle damage led to extensive calcification and deposition of adipose and fibrotic tissues with the appearance of rare regenerated myofibers. Importantly, analysis of Pax7 & minus;/ & minus; muscle suspensions indicated that myogenic cells expressing Pax3 and MyoD were responsible for this low level of regeneration.