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Growth Rate and Nutrient Uptake Rate Comparison Between C8161 Cells and Hybridoma Cells

出版	University of Louisville, 2003
URL	http://books.google.com.hk/books?id=mI4VtwAACAAJ&hl=&source=gbs_api

註釋Many pharmaceutical products can be created from genetically engineered mammalian cells grown within a bioreactor. The products formed include proteins, such as certain hormones and antibodies, and some vaccines, all of which are currently used for diagnosis and treatment of many diseases, with the potential to treat many others. Because of this potential, there is a large demand for therapeutic proteins. With this demand comes the need for large-scale, cost effective bioreactors in which to grow the cells and synthesize product. The C8161 cell line was isolated from highly aggressive cutaneous melanoma. This cell line creates its own vascular-like network in vitro. If the cells create their vascular structure within a bioreactor, a tissue dense system can be created, reducing the size requirements of the bioreactor, and resulting in lower startup and operating costs. Also, the C8161 cell line is mammalian, eliminating the problems associated with bacterial cultures. Three parameters were tested to begin the process of characterizing the C8161 cell line for use within a bioreactor: specific growth rate, glucose uptake rates, and oxygen uptake rates. The specific growth rate, glucose uptake rate, and oxygen uptake rate were calculated to be 0.014/hour, 5.18 x 10−10 mmol glucose/cell/hour, and 1.23 x 10−13 mole O2/cell/hour, respectively. These values were similar to hybridoma cells currently being used in bioreactors. From the experimental results it was determined that the C8161 cell line would be a good candidate for use within a bioreactor environment.