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Uncontrolled Diabetes Mellitus Has No Major Influence on the Platelet Transcriptome

Thomas NührenbergMarco Alexander CederqvistFederico MariniChristian W. StratzBjörn GrüningDietmar TrenkHarald BinderRalf GilsbachFranz-Josef NeumannLutz Hein

出版	Universität, 2018
URL	http://books.google.com.hk/books?id=mqmRzgEACAAJ&hl=&source=gbs_api

註釋Abstract: Background. Diabetes mellitus (DM) has been associated with increased platelet reactivity as well as increased levels of platelet RNAs in plasma. Here, we sought to evaluate whether the platelet transcriptome is altered in the presence of uncontrolled DM. Methods. Next-generation sequencing (NGS) was performed on platelet RNA for 5 patients with uncontrolled DM (HbA1c 9.0%) and 5 control patients (HbA1c 5.5%) with otherwise similar clinical characteristics. RNA was isolated from leucocyte-depleted platelet-rich plasma. Libraries of platelet RNAs were created separately for long RNAs after ribosomal depletion and for small RNAs from total RNA, followed by next-generation sequencing. Results. Platelets in both groups demonstrated RNA expression profiles characterized by absence of leukocyte-specific transcripts, high expression of well-known platelet transcripts, and in total 6,343 consistently detectable transcripts. Extensive statistical bioinformatic analysis yielded 12 genes with consistently differential expression at a lenient FDR