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Immunocytochemical Studies on Peptidergic Neurons in the Colorado Potato Beetle and Some Other Insect Species
Jan Adrianus Veenstra
出版
J.A. Veenstra
, 1984
URL
http://books.google.com.hk/books?id=nNdMAAAAYAAJ&hl=&source=gbs_api
註釋
In chapter five, a large number of antisera to various regulatory vertebrate peptides was tested on the Colorado potato beetle, to reveal peptidergic neurons and neurosecretory cells and to differentiate between these cells. Immunoreactive cells were revealed by antisera to ACTH, gastrin, cholecystokinin, alpha-endorphin, beta-endorphin, gammal-MSH, insulin, human calcitonin, motilin, growth hormone, somatostatin, corticotropin-releasing factor, ovine prolactin and rat prolactin. Some of these immunoreactive cells seem to function as neurons, whereas others function as neurosecretory cells. It is postulated that if the part of the substance recognized by the antiserurn is of physiological importance to the insect, that part is retained in several species. The antiserurn should then reveal homologous neurons in different insect species. Given the fact that insect species differ widely in their immunocytochemical responses, the criterion of staining of probably homologous neurons offers some help in separating relevant from irrelevant immunoreactions. The immunocytochemical data are evaluated according to this concept.