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Identification and Characterisation of the Equine Morbillivirus C Protein

Terry Sunderland

出版	The Author, 1997
URL	http://books.google.com.hk/books?id=qDTfNAAACAAJ&hl=&source=gbs_api

註釋

Summary: Equine morbillivirus (EMV) emerged dramatically, in two unrelated outbreaks, resulting in the death of two humans and 16 horses. This virus represents a new member of the family paramyxoviridae, is different from any existing members and is classified as a biosecurity level 4 agent, as for the Ebola virus. The C protein, considered a non-structural protein, is translated from the P and V mRNAs in a separate overlapping reading frame. Observations of paramyxovirinae C proteins have been inconsistent, with no clear function for this protein being elucidated. This has necessitated the investigation of the EMV C protein. To achieve this the C gene was cloned from a total EMV cDNA library and expressed in bacteria, an insect cell line and a mammalian system. The recombinant protein was purified and used to raise hyper-immune sera in rabbits. The sera produced enabled us to demonstrate the presence of the C protein not only in EMV infected cell lysate, but also within purified virions. This study will aid in the reclassification of EMV and provide additional information regarding the role of the C protein.