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Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Sergio Colonna-RomanoAntonella LeoneBruno Maresca
出版Springer Science & Business Media, 2012-12-06
主題Science / Life Sciences / Molecular BiologyScience / Life Sciences / BiochemistryScience / Life Sciences / Cell BiologyScience / Life Sciences / Microbiology
ISBN36428045439783642804540
URLhttp://books.google.com.hk/books?id=qFHuCAAAQBAJ&hl=&source=gbs_api
EBookSAMPLE
註釋Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.